Coding

Part:BBa_K929200:Design

Designed by: Potsdam Bioware 2012 iGEM   Group: iGEM12_Potsdam_Bioware   (2012-09-20)

N-terminal sortase motif linked to AAV2-VP2 with Myc-tag, kozak and CMV


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2060
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2696
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2619
    Illegal SapI site found at 1497


Design Notes

Primer (forward) with XbaI restriction site, kozak consensus sequence, Sortase motif and myc tag:

Prf + myc.png

Primer (reverse, complement) with PstI restriction site:

Prr.png


Proceeding


UP12 Sortase myc.png

Source

pCMV_DARPin-E01_Middle-Linker_[AAV2]-VP23 (ViralBrick-587KO-Empty) (BBa_K404161) and self-designed primers

References